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Colony characteristics
Candida albicans
Aspergillus flavus
Aspergillus flavus
Yeast like colonies with feet (projections) on chocolate agar
Aspergillus flavus
Aspergillus flavus
Aspergillus flavus
Yellowish-green colonies, consisting of a dense felt of conidiophores
Exophiala dermatitidis
Exophiala dermatitidis
Exophiala dermatitidis
Smooth and waxy colonies with brown pigment exuded into the agar.
Histoplasma capsulatum
Exophiala dermatitidis
Exophiala dermatitidis
Granular to cottony colonies, initially white/cream colored, later becoming brownis with age
Microscopy
Aspergillus fumigatus
Aspergillus fumigatus
Aspergillus fumigatus
Vesicles subclavate with uniseriate conidiogenous cells. 40x magnification.
Petriella setifera
Aspergillus fumigatus
Aspergillus fumigatus
Cylindrical conidiogenous cells morphologically matching the genus Scedosporium, hyaline, obovoidal conidia. 20x magnification.
Rhizopus species
Aspergillus fumigatus
Rhizopus species
Unbranched sporangiophores, rhizoids, and columella . 40x magnification.
Direct Microscopy-Gram stain
Sputum
Spherules.100x magnification. Coccidioides immitis/posadasii.
Blood
Yeast like cells with pseudohyphae . 100x magnification. Candida albicans.
CSF
CSF
Large size budding yeasts. Capsule not seen. 100x magnification. Cryptococcus neoformans.
Direct Microscopy-Fluorescent stain
Wound
Branching septate Hyphae. 50x magnification. Aspergillus nidulans.
Biopsy Lymph Node
Yeast cells. 50x magnification. Histoplasma capsulatum.
Abscess
Abscess
Septate Hyphae. 50x magnification. Scedosporium apiospermum.
Antifungal suceptiility testing
Yeast
Yeast
Yeast
Colorimetric method
Mold
Yeast
Yeast
Microbroth Dilution